《植物生理学报》 2009, 45(10): 967-971

丁烯羟酸内酯对公鸡花种子萌发和赤霉素含量的影响

周俊芳^1,2, 马国华^1,*

1 中国科学院华南植物园, 广州510650; 2 中国科学院研究生院, 北京100049

通信作者：马国华；E-mail: magh@scib.ac.cn；Tel: 020-37252993

摘　要：

从植物燃烟中提取的丁烯羟酸内酯(butenolide)能提高公鸡花种子的萌发率, 并促进其萌发后的生长。光可以增强丁 烯羟酸内酯的作用。公鸡花对外源GA的响应与对丁烯羟酸内酯的响应类似, 二者有协同效应。酶联免疫法测定结果显示, 在种子尚未萌发时, 经丁烯羟酸内酯处理的种子中内源赤霉素含量低于不做处理的; 萌发生长后, 其赤霉素含量则高于不 做处理的。因此认为, 丁烯羟酸内酯可能是通过增加种子对 GA 的敏感性而起作用的。

关键词：丁烯羟酸内酯; 光; 赤霉素; 种子萌发; 公鸡花

收稿：2009-07-10   修定：2009-09-27

资助：国家自然科学基金(30671711)。

Effects of Butenolide on Seed Germination and Endogenous Gibberellin Contents in Aristolochia labiata Willd.

ZHOU Jun-Fang^1,2, MA Guo-Hua^1,*

¹South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; ²Graduate University of Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: MA Guo-Hua; E-mail: magh@scib.ac.cn; Tel: 020-37252993

Abstract:

Smoke-derived butenolide could increase the rate of Aristolochia labiata seed germination and improve their post-germination growth. Light could enhance the effects of butenolide. Seed had similar response to gibberellins and butenolide, and they had a synergetic effect. Enzyme-linked immunosorbent assays (ELISA) revealed that the gibberellin levels of seeds treated with butenolide were significantly less than controls when germination was not finished; however, gibberellin contents were higher than controls after germination. These results suggested that butenolide treatment could increase sensitivity to GA.

Key words: butenolide; light; GA; seed germination; Aristolochia labiata